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Team:UNICAMP-Brazil/Protocols/Restriction reaction
From 2009.igem.org
Restriction Reaction
- 1X Reaction Buffer
- Total DNA - 0.1-4 μg
- Endonuclease - 1 unit per μg of DNA
- Autoclaved distilled water to 20 uL
Temperature - 37°C - or the suggested by the manufacturer
Time - 3 h or overnight
Protocol according to Current Protocols in Molecular Biology
