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From 2009.igem.org

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	LB medium DNA resuspension from iGEM 2009 plates Transformation X-Gal staining protocol for beta galactosidase (blue/white screening) Glycerol stocks Plasmid extraction BioBrick digestion with restriction enzymes Electrophoresis DNA gel extraction DNA precipitation with sodium acetate Ligation PCR Sensing ethanol concentration: our do-it-yourself kit

DNA gel extraction

(Estimated time: 1 hour and 30 min)

Follow Roche standard protocol: Roche Agarose Gel DNA Extraction Kit protocol

INGREDIENTS: Agarose gel with well-separated, sharp bands Scalpel and tweezers to cut gel (they have to be cleaned in ddH2O after every cut) Roche Agarose Gel DNA Extraction Kit

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