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Future Work
- Resolve problems with conversion of broad-host range vectors to BioBrick compatible formats.
- Further test the broad-host vectors to verify their functionality in additional organisms.
- Further test different GFP and phasin constructs and determine additional ways to monitor phasin production, such as by mass spectrometry or transmission electron microscopy.
- Design additional signal peptides that are functional with organisms like Synechocystis PCC6803, P. putida, and R. sphaeroides, and that can be expressed in BioBrick-compatible broad-host range vectors.
- Optimize and expand both systems to include different protein products and organisms.
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