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Team:Virginia/10 June 2009
From 2009.igem.org
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Overnight Results
- Only saw growth in pSB1C3 broth
- Have colonies on A09-1(2) plate
Today
- Made 5ml overnight broths from 3 colonies on A09-1(2)
- Miniprepped pSB1C3
- 18.4 ng/ul
- Re-transformed vector DNA in DB3.1
- included control transformation in DH5a, should yield no colonies
- Prepared -20 glycerol stocks of knockouts JW3469 and JW3470.
