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Team:Virginia/11 August 2009
From 2009.igem.org
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Overnight Results
- Small broths with ABP had no visible growth, but those with pSB3K3, PCS, and ABP-HA(2-5) had growth
Today
- Miniprep of pSB3K3, PCS, and ABP-HA(2-5)
- Sent ABP-HA(2-5) to be sequenced in Jordan with VF2/VR primers
- Gel of PCR products from 76/82 samples using RFP/GFP/rRNA primers
- Growth curve in 0.2mM As
- Using cuvette growing technique
- Transformed and plated A09-11 (ArsR + E0240 in pSB1A2)
- Cut pSB3k3 E,P
- Gel to be added
