Team:Virginia/12 August 2009


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Overnight Results

  • A09-11 had colonies and satellite colonies


  • Ligated and transformed A09-12 (pSB3K3 + ArsR + E0240)
  • New PCR to add HA to PCS. Not using a separated cycle instead running 29 cycles at 65C annealing temp
  • DNA concentrations of gel extracts of fragments not good enough, hence:
  • Amplifying more product for RFP, GFP and rRNA fragments