"
Team:Virginia/15 June 2009
From 2009.igem.org
Home || Back to Notebook || Upload image
Overnight Results
- Plate of A09-2 dilute (PRE BFP) had colonies
- Still not seeing visible BFP
Today
- Ordered Arsenic binding peptide (ABP) as pair of oligos
- BB prefix & suffix
- Two repeats of CCGCG (Cys-Cys-Gly-Cys-Gly) with Lys-Gly-His linkers after each
- Complete AA sequence: CCGCGKGHCCGCGKGH
- Complete DNA sequence: gaattcgcggccgcttctagATGTGTTGTGGTTGCGGTAAAGGTCATTGTTGTGGTTGTGGTAAAGGTCATtactagtagcggccgctgcag
- Getting quote for PCS in DNA 2.0's pJ express in-house expression vector.
- with BB suffix, no BB prefix
- Can add the prefix later with PCR, but its stop codon would interfere with expression
