Team:Virginia/4 August 2009


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  • Miniprep of ABP / ABP+HA in vectors
    • Conc: 17.8ng/ul and 33.3ng/ul, respectively
  • Digest of above (E,P)
    • Gel:
  • New PCR to HA to ABP again
    • Temps: 54C for 6 cycles for overlap part to bind, then 60C for 24 cycles (calculated salt-adjusted temp for long primer is 87C, shorter one is 63C)