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Team:Wash U/Calendar-Home/1 July 2009
From 2009.igem.org
-Miniprep #2 to obtain more biobrick DNA
-New gel with digested vs. undigested plasmids
-Started new restriction digests on plasmids (single cut with enzyme #1, single cut with enzyme #2, double cut); will run these on gel tomorrow
-Started new overnight cultures of biobrick parts and plasmids
-Team meeting #4
Miniprep #2:
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1) BBa_I15010 pSB2K3 2) BBa_E0240 pSB1A2 3) BBa_R0082 pSB1A2 4) plasmid pSB1T3 5) plasmid pSB1K3 6) BBa_B0034 pSB1A2 7) plasmid pSB1AT3 8) plasmid pSB1A3 9) plasmid pucHS27 10) plasmid pSB1AC3 11) BBa_B0015 pSB1AK3 12) plasmid pRKCBC3 |
35 ng/ul 45 ng/ul used 62.5 ng/ul 42 ng/ul 28.5 ng/ul 133.0 ng/ul 62.5 ng/ul 54.0 ng/ul 64.5 ng/ul 31.5 ng/ul 45.5 ng/ul |
Note: Nanospec: Used 3ul on Lid 10, 320nm background ON, blank after each measurement with TE elution buffer (Sigma) *Measured again with EtOH wipe after each measurement 7-15
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