Miniprep:
Sample 1 term C 2 term B 3 pG 4 pH 5 pE 6 pF 7 pC 8 pD 9 pA 10 pB 11 term A 12 cph8 D 13 cph8 B 14 cph8 C 15 term D 16 cph8 A 17 ompC+p C 18 ompC+p B 19 ompC+p A
| ng/ul
100 207 98 90 57 75 48.5 67.5 57 44.5 155 32.5 41 37 151 34.5 92 109 93.5 |
-Diagnostic Digest of all samples
-Gel purified Cph8 PCR
-Discovered PstI cut site in newly acquired Cph8; now using prefix Silver ligation