Team:Waterloo/Notebook/Protocols/Glycerol Stock Preparation


Glycerol Stock Preparation

Before you start
- It is important to use aseptic technique when carrying out the procedures

5 mL culture tube
60% Glycerol
Sterile screw-top cryotube

1. Inoculate a 5 mL culture tube.
2. Incubate overnight on the shaker.
3. Pipette 500 μL of 60% glycerol culture into a sterile screw-top cryotube.
4. Piptette 500 μL of culture into the cryotube.
5. Label and date cryotube.
6. Vortex.
7. Cover the label with clear tape.
8. Store in -80°C freezer.
9. Add strain to database.