Team:Wisconsin-Madison/Colony Polymerase Chain Reaction (PCR)

From 2009.igem.org



Colony PCR

Pick a colony and streak a small patch on a plate (that way you can use one plate for all of your screening) and then pipette with the same tip up and down several times into one of the 10uL aliquots. Make sure you also run a positive and negative control.

Materials

  • 12.5 μL Master Mix (Go Taq)
  • 11 μL DNA in H20
  • 0.5 μL Fwd Primer
  • 0.5 μL Rev Primer


  • Thermocycler
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