Team:Wisconsin-Madison/Ligation

Ligation

Concentration

• 1 backbone : 5 insert
• insert: [100 x (insert size bp/backbone size bp) x 5 / concentration(ng)] μL

Thermocycler

• users: Dan A: Lig
• 2 hours 16 C or overnight

Materials

• 1μL T4 ligase
• 1 μL T4 ligase buffer
• backbone + insert =< 8μL (remainder H20)