Team:Wisconsin-Madison/Restriction Digestion

From 2009.igem.org



Restriction Enzyme Mapping

Use smallest micro cuvettes and mix (total volume 18 μL):

  • 1.8 μL Buffer (10x)
  • 0.18 μL BSA +3 (100x)
  • 12 μL MiliQ H20
  • 3 μL DNA
  • 1 μL Enzyme (100x)

Incubate in water bath at 37 C for 2 hours:

  • 3.6 μL Dye (6x)


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