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Template:Team:KULeuven/10 September 2009/VanillinProduction
From 2009.igem.org
- Gel electrophoresis of TER restriction
- The vector for the TER is Psb1AK3 and has 3100bp, with an insert of ±3200 bp
- Gel extraction
| part | concentration | 260/280 | 260/230 |
|---|---|---|---|
| TER | 14,2 | 1,97 |
- Created a motherplate from EF1
- Ligated EF restriction + TER restriction
- Transferred the SAMS 1 and SAMS 2 colonies to liquid medium
