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Template:Team:KULeuven/12 August 2009/VanillinProduction
From 2009.igem.org
- Miniprep on sam5, sam8 and fcs
- There was no growth on ech, thus new cultures in liquid medium
- Restriction digest on sam5, sam8 and fcs
- Gel electrophoresis on sam5, sam8 and fcs
- Gel extraction on sam5 and fcs
Nanodrop results:
| Part | concentration (ng/μl) | 260/280 λ | 260/230 λ |
|---|---|---|---|
| fcs | 4,2 | 6,08 | |
| sam5 | 7,3 | 2,76 |
Notes:
- sam8 showed a very low concentration on the gel electrophoresis. Thus, it is better to redo this test.
- 260/280 for fcs was very high and the concentration was not high enough. Therefore, we will redo the extraction and use multiple tubes on the same filter to increase the concentration
