Template:Team:KULeuven/18 October 2009/VanillinProduction

From 2009.igem.org

  • Vanilla experiment take 3!
    • Transfered 1:100 to new LB cultures containing tyrosine or ferrulic acid or vanillin
      • All had a concentration of 5 mg/L, vanillin was used to determine if vanillin remained stabile in e.coli (or perhaps was converted to another product)
      • Ferrulic acid and vanillin were dissolved in alcohol at 10 mg/ml

Resulting experiments:

  1. EFT+ Vanillin
  2. PEFT + Ferrulic accid (2x)
  3. EFT + Ferrulic acid (2)
  4. PAMSCOT + Tyrosine (2x)
  5. SAMS + Tyrosine (2x)
  • At the start of the experiment, samples were taken
  • The next day the samples were taken to the HPLC for measurements.
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