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Template:Team:KULeuven/18 September 2009/VanillinProduction
From 2009.igem.org
- Miniprep EFT
- Restriction digest EFT with X/P
- Gel electrophoresis EFT for 1,5 hour, because the difference between insert (2600bp) and vector (3100bp) is small.
- EFT is present in all 4 samples
- Gel extraction of EFT
- Ligation of the gel extract and the promotor , and ligation of the non-gel extract and the promotor.
- Transfered 6 colonies of PAMS to a new plate and on liquid culture.
