Template:Team:KULeuven/18 September 2009/VanillinReceptor

From 2009.igem.org

  • We checked if the PstI had cut the mutagenated places by gel-electrophoresis but we forgot to add a sample as positive control so the restriction digest was done again. The result we put on gel and saw that there was no cutting in both samples. Restriction disgest with pstI was now done overnight.
  • virB ligation showed good results so it was electroporated