"
Template:Team:KULeuven/19 September 2009/VanillinProduction
From 2009.igem.org
- Miniprep PAMS
- Restriction digest PAMS with S/P
- Restriction digest COMT 13, 11, 21, 31 , 41, 42 with X/P
- Ligation PAMS&CO (Promotor, Sam5, Sam8, ComT)
- Again one part from gel extraction (UV)
- One part directly after restriction digest
- Electroporation PEFT and PEFT (UV), both twice.
