Template:Team:KULeuven/2 September 2009/VanillinProduction
From 2009.igem.org
- The four grown colonies are put on liquid culture
- New (again...) restriction digest on sam5, sam8, fcs and ech, genes were miniprepped on Tuesday.
- Overnight at 16°C
- The plates with sam5, sam8, ech and fcs were re-ented. From sam5 and fcs four colonies were picked and plated.
- The PCR from yesterday did not deliver any results, probably because of an error when preparing the PCR mix. A new PCR was prepared and started today.