Template:Team:KULeuven/2 September 2009/VanillinProduction

From 2009.igem.org

  • The four grown colonies are put on liquid culture
  • New (again...) restriction digest on sam5, sam8, fcs and ech, genes were miniprepped on Tuesday.
    • Overnight at 16°C
  • The plates with sam5, sam8, ech and fcs were re-ented. From sam5 and fcs four colonies were picked and plated.
  • The PCR from yesterday did not deliver any results, probably because of an error when preparing the PCR mix. A new PCR was prepared and started today.