Template:Team:KULeuven/31 August 2009/VanillinReceptor

From 2009.igem.org

  • TOPO cloning showed only blue colonies so we searched for alternatives. We did resaerchwork if it was maybe possible with a puc-vector
  • The mutation of rpoA was again checked but gelelectrophoresis showed no result
  • X,Y and K (the igemvector) were cut with EcoR1 and Pst1.
  • new ligation was done with the first cuttingproduct so today it was electroporated and plated.
  • PCR was done for W to get more basicproduct to work with
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