Template:Team:KULeuven/7 September 2009/BlueLightReceptor

From 2009.igem.org

  1. Restriction digest with EcoRI and PstI on and the purified pcr product of in order to get our promotor on itself in the standard vector.
  2. Gel electroforesis and extraction of this RD. The concentration of this extraction was too low so no ligation was performed with these samples.
  3. Extra pcr was done on with primers 2260 and 2261.
  4. Liquid cultures of LigX and of were made from the 4x plates.