Template:Team:KULeuven/7 September 2009/VanillinProduction
From 2009.igem.org
- Miniprep of the four colonies from EF ligation
- Accidentally used double volumes of elution buffer on EF4
| part | concentration | 260/280 | 260/230
|
| EF 1 | 115,5 | 1,98 | 2,31
|
| EF 2 | 105,2 | 1,96 | 1,66
|
| EF 3 | 95,1 | 2,05 | 0,98
|
| EF 4 | 52,7 | 2,06 | 0,97
|
- Gel electrophoresis from restriction on sam5 and sam8 looked good
- Purified and ligated the restriction from sam5 and sam8 overnight
- Concentration of sam8 was barely enough for ligation...
| part | concentration | 260/280 | 260/230
|
| sam5 | 10,1 | 2,11 | 0,04
|
| sam8 | 3,1 | 3,96 | 0,02
|
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