UCL London/Protocol/Gel Electrophoresis

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Gel Electrophoresis

Materials:

• Agarose
• TAE buffer
• TAE buffer

Method:

1. Weigh 1.50g agarose, and add 1×TAE to make up to 150mL.
2. Cool down the agarose and add 15 µL ethidium bromide into the agrose.
3. Pour the gel into the tray before it settles down.
4. Spin the digested DNA for 5 sec.
5. Add 5 µL orange loading buffer (glycerol & dye) to the lid; spin for few sec.
6. Cover the gel by TAE.
7. Load the gel and note down the sequence.
8. At 110V, running for 30 min (or 1hr depends on the equipment).
9. Check with UV light & take picture.