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September M T W T F S S   [http://2009.igem.org/ULB/1_September_2009 1] [http://2009.igem.org/wiki/index.php?title=ULB/2_September_2009&action=edit 2] [http://2009.igem.org/ULB/3_September_2009 3] [http://2009.igem.org/ULB/4_September_2009 4] [http://2009.igem.org/ULB/5_September_2009 5] [http://2009.igem.org/wiki/index.php?title=ULB/6_September_2009&action=edit 6] [http://2009.igem.org/ULB/7_September_2009 7] [http://2009.igem.org/wiki/index.php?title=ULB/8_September_2009&action=edit 8] [http://2009.igem.org/ULB/9_September_2009 9] [http://2009.igem.org/ULB/10_September_2009 10] [http://2009.igem.org/ULB/11_September_2009 11] [http://2009.igem.org/ULB/12_September_2009 12] [http://2009.igem.org/wiki/index.php?title=ULB/13_September_2009&action=edit 13] [http://2009.igem.org/ULB/14_September_2009 14] [http://2009.igem.org/wiki/index.php?title=ULB/15_September_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=ULB/16_September_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=ULB/17_September_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=ULB/18_September_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=ULB/19_September_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=ULB/20_September_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=ULB/21_September_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=ULB/22_September_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=ULB/23_September_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=ULB/24_September_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=ULB/25_September_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=ULB/26_September_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=ULB/27_September_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=ULB/28_September_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=ULB/29_September_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=ULB/30_September_2009&action=edit 30]

Contents 1 July 12, 2009 1.1 Primers design for hfsG et hfsH genes 1.1.1 BBa_R0011 (promoter) : 1.1.2 RBS BBa_B0034 : 1.1.3 hfsG gene : 1.1.4 hfsH gene : 1.1.5 RBS + RFP : 1.1.6 BBa_B0015 terminator : 1.2 Sequences 1.2.1 Promoter (55 bp) 1.2.2 RBS (12 pb) 1.2.3 RBS + RFP (681 bp) 1.2.4 Terminator (129 bp) 1.2.5 Plasmid (3165 bp)

July 12, 2009

Forward and reverse primers are designed and ordered.

Primers design for hfsG et hfsH genes

BBa_R0011 (promoter) :

PREFIX :

=> GAATTCATGAGATCT aattgtgagcggataaca

SUFFIX :

<= ctcgagttaggatcc TGTGCTCAGTATCTTGTT

RBS BBa_B0034 :

PREFIX :

=> GAATTCATGAGATCT AAAGAG

SUFFIX :

<= ctcgagttaggatcc TTTCTC

hfsG gene :

PREFIX :

=> GAATTCATGAGATCT atgaacgcgcccgtcaacgagctgcgactggagaacgccg

SUFFIX :

<= cagacggcctcgctgtaga

hfsH gene :

PREFIX :

=> atgccgatggagttcgagaag

SUFFIX :

<= ctcgagttaggatcc ttagagcccgatcc

RBS + RFP :

PREFIX :

=> GAATTCATGAGATCT aaagaggagaaatactag

SUFFIX :

<= ctcgagttaggatcc GCGATCTACACTAGCACT

BBa_B0015 terminator :

PREFIX :

=> GAATTCATGAGATCT ccaggcatcaaataaaac

SUFFIX :

<= ctcgagttaggatcc TATAAACGCAGAAAGGCC

Sequences

Promoter (55 bp)

aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca

RBS (12 pb)

aaagaggagaaa

RBS + RFP (681 bp)

aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgt
atggaaggttccgttaacggtcacgagttcgAaatcgaaggtgaaggtgaaggtcgtccgtacgaaggt
acccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccg
cagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtcc
ttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccag
gactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgac
ggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggt
gctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaa
accacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatc
acctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggt
gcttaataacgctgatagtgctagtgtagatcgc

Terminator (129 bp)

ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata

Plasmid (3165 bp)

ggatcctaactcgaggttacattgtcgatctgttcatggtgaacagctttaaatgcaccaaaaactcgt
aaaagctctgatgtatctatcttttttacaccgttttcatctgtgcatatggacagttttccctttgat
atctaacggtgaacagttgttctacttttgtttgttagtcttgatgcttcactgatagatacaagagcc
ataagaacctcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgtgag
ccatgagaacgaaccattgagatcatgcttactttgcatgtcactcaaaaattttgcctcaaaactggt
gagctgaatttttgcagttaaagcatcgtgtagtgtttttcttagtccgttacgtaggtaggaatctga
tgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggttacgagatc
catttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaccaccaa
tttcatattgctgtaagtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaa
ctcatggtagttattttcaagcattaacatgaacttaaattcatcaaggctaatctctatatttgcctt
gtgagttttcttttgtgttagttcttttaataaccactcataaatcctcatagagtatttgttttcaaa
agacttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttc
actaaaaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagcc
tttaaccaaaggattcctgatttccacagttctcgtcatcagctctctggttgctttagctaatacacc
ataagcattttccctactgatgttcatcatctgagcgtattggttataagtgaacgataccgtccgttc
tttccttgtagggttttcaatcgtggggttgagtagtgccacacagcataaaattagcttggtttcatg
ctccgttaagtcatagcgactaatcgctagttcatttgctttgaaaacaactaattcagacatacatct
caattggtctaggtgattttaatcactataccaattgagatgggctagtcaatgataattacatgtcct
tttcctttgagttgtgggtatctgtaaattctgctagacctttgctggaaaacttgtaaattctgctag
accctctgtaaattccgctagacctttgtgtgttttttttgtttatattcaagtggttataatttatag
aataaagaaagaataaaaaaagataaaaagaatagatcccagccctgtgtataactcactactttagtc
agttccgcagtattacaaaaggatgtcgcaaacgctgtttgctcctctacaaaacagaccttaaaaccc
taaaggcttaagtagcaccctcgcaagctcgggcaaatcgctgaatattccttttgtctccgaccatca
ggcacctgagtcgctgtctttttcgtgacattcagttcgctgcgctcacggctctggcagtgaatgggg
gtaaatggcactacaggcgccttttatggattcatgcaaggaaactacccataatacaagaaaagcccg
tcacgggcttctcagggcgttttatggcgggtctgctatgtggtgctatctgactttttgctgttcagc
agttcctgccctctgattttccagtctgaccacttcggattatcccgtgacaggtcattcagactggct
aatgcacccagtaaggcagcggtatcatcaacaggcttacccgtcttactgtccctagtgcttggattc
tcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcatt
actggatctatcaacaggagtccaagcgagctcgtaaacttggtctgacagttaccaatgcttaatcag
tgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagat
aactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcacc
ggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaacttt
atccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagttt
gcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcag
ctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctcctt
cggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgca
taattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcatt
ctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccaca
tagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttacc
gctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcac
cagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaa
atgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgag
cggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttcccatggtgcc
acctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaa
tttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcatgagatct

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