Week12

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1.FACS with Host as Control .

2.We got one new host strain which is lacI deleted at the same time both kanamycin and ampicillin sensitive.

3.Competent cells were prepared from this strain.

4.Transformations done with new host strain E.coli MG1655- some problem with ampicillin plates. There was some

colonies on the control plate also. Problem thought was that there may be some contamination while preparing

competent cells for the new hosts.