Wisconsin-Madison/11 June 2009

From 2009.igem.org

Calendar

April
MTWTFSS
    [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/1_April_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/2_April_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/3_April_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/4_April_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/5_April_2009&action=edit 5]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/6_April_2009&action=edit 6] [http://2009.igem.org/Wisconsin-Madison/7_April_2009 7] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/8_April_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/9_April_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/10_April_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/11_April_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/12_April_2009&action=edit 12]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/13_April_2009&action=edit 13] [http://2009.igem.org/Wisconsin-Madison/14_April_2009 14] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/15_April_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/16_April_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/17_April_2009&action=edit 17] [http://2009.igem.org/Wisconsin-Madison/18_April_2009 18] [http://2009.igem.org/Wisconsin-Madison/19_April_2009 19]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/20_April_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/21_April_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/22_April_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/23_April_2009&action=edit 23] [http://2009.igem.org/Wisconsin-Madison/24_April_2009 24] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/25_April_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/26_April_2009&action=edit 26]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/27_April_2009&action=edit 27] [http://2009.igem.org/Wisconsin-Madison/28_April_2009 28] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/29_April_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/30_April_2009&action=edit 30]
May
MTWTFSS
        [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/1_May_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/2_May_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/3_May_2009&action=edit 3]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/4_May_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/5_May_2009&action=edit 5] [http://2009.igem.org/Wisconsin-Madison/6_May_2009 6] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/7_May_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/8_May_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/9_May_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/10_May_2009&action=edit 10]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/11_May_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/12_May_2009&action=edit 12] [http://2009.igem.org/Wisconsin-Madison/13_May_2009 13] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/14_May_2009&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/15_May_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/16_May_2009&action=edit 16] [http://2009.igem.org/Wisconsin-Madison/17_May_2009 17]
[http://2009.igem.org/Wisconsin-Madison/18_May_2009 18] [http://2009.igem.org/Wisconsin-Madison/19_May_2009 19] [http://2009.igem.org/Wisconsin-Madison/20_May_2009 20] [http://2009.igem.org/Wisconsin-Madison/21_May_2009 21] [http://2009.igem.org/Wisconsin-Madison/22_May_2009 22] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/23_May_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/24_May_2009&action=edit 24]
[http://2009.igem.org/Wisconsin-Madison/25_May_2009 25] [http://2009.igem.org/Wisconsin-Madison/26_May_2009 26] [http://2009.igem.org/Wisconsin-Madison/27_May_2009 27] [http://2009.igem.org/Wisconsin-Madison/28_May_2009 28] [http://2009.igem.org/Wisconsin-Madison/29_May_2009 29] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/30_May_2009&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/31_May_2009&action=edit 31]
June
MTWTFSS
[http://2009.igem.org/Wisconsin-Madison/1_June_2009 1] [http://2009.igem.org/Wisconsin-Madison/2_June_2009 2] [http://2009.igem.org/Wisconsin-Madison/3_June_2009 3] [http://2009.igem.org/Wisconsin-Madison/4_June_2009 4] [http://2009.igem.org/Wisconsin-Madison/5_June_2009 5] [http://2009.igem.org/Wisconsin-Madison/6_June_2009 6] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/7_June_2009&action=edit 7]
[http://2009.igem.org/Wisconsin-Madison/8_June_2009 8] [http://2009.igem.org/Wisconsin-Madison/9_June_2009 9] [http://2009.igem.org/Wisconsin-Madison/10_June_2009 10] [http://2009.igem.org/Wisconsin-Madison/11_June_2009 11] [http://2009.igem.org/Wisconsin-Madison/12_June_2009 12] [http://2009.igem.org/Wisconsin-Madison/13_June_2009 13] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/14_June_2009&action=edit 14]
[http://2009.igem.org/Wisconsin-Madison/15_June_2009 15] [http://2009.igem.org/Wisconsin-Madison/16_June_2009 16] [http://2009.igem.org/Wisconsin-Madison/17_June_2009 17] [http://2009.igem.org/Wisconsin-Madison/18_June_2009 18] [http://2009.igem.org/Wisconsin-Madison/19_June_2009 19] [http://2009.igem.org/Wisconsin-Madison/20_June_2009 20] [http://2009.igem.org/Wisconsin-Madison/21_June_2009 21]
[http://2009.igem.org/Wisconsin-Madison/22_June_2009 22] [http://2009.igem.org/Wisconsin-Madison/23_June_2009 23] [http://2009.igem.org/Wisconsin-Madison/24_June_2009 24] [http://2009.igem.org/Wisconsin-Madison/25_June_2009 25] [http://2009.igem.org/Wisconsin-Madison/26_June_2009 26] [http://2009.igem.org/Wisconsin-Madison/27_June_2009 27] [http://2009.igem.org/Wisconsin-Madison/28_June_2009 28]
[http://2009.igem.org/Wisconsin-Madison/29_June_2009 29] [http://2009.igem.org/Wisconsin-Madison/30_June_2009 30]
July
MTWTFSS
    [http://2009.igem.org/Wisconsin-Madison/1_July_2009 1] [http://2009.igem.org/Wisconsin-Madison/2_July_2009 2] [http://2009.igem.org/Wisconsin-Madison/3_July_2009 3] [http://2009.igem.org/Wisconsin-Madison/4_July_2009 4] [http://2009.igem.org/Wisconsin-Madison/5_July_2009 5]
[http://2009.igem.org/Wisconsin-Madison/6_July_2009 6] [http://2009.igem.org/Wisconsin-Madison/7_July_2009 7] [http://2009.igem.org/Wisconsin-Madison/8_July_2009 8] [http://2009.igem.org/Wisconsin-Madison/9_July_2009 9] [http://2009.igem.org/Wisconsin-Madison/10_July_2009 10] [http://2009.igem.org/Wisconsin-Madison/11_July_2009 11] [http://2009.igem.org/Wisconsin-Madison/12_July_2009 12]
[http://2009.igem.org/Wisconsin-Madison/13_July_2009 13] [http://2009.igem.org/Wisconsin-Madison/14_July_2009 14] [http://2009.igem.org/Wisconsin-Madison/15_July_2009 15] [http://2009.igem.org/Wisconsin-Madison/16_July_2009 16] [http://2009.igem.org/Wisconsin-Madison/17_July_2009 17] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/18_July_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/19_July_2009&action=edit 19]
[http://2009.igem.org/Wisconsin-Madison/20_July_2009 20] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/21_July_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/22_July_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/23_July_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/24_July_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/25_July_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/26_July_2009&action=edit 26]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/27_July_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/28_July_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/29_July_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/30_July_2009&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/31_July_2009&action=edit 31]
August
MTWTFSS
          [http://2009.igem.org/Wisconsin-Madison/1_August_2009 1] [http://2009.igem.org/Wisconsin-Madison/2_August_2009 2]
[http://2009.igem.org/Wisconsin-Madison/3_August_2009 3] [http://2009.igem.org/Wisconsin-Madison/4_August_2009 4] [http://2009.igem.org/Wisconsin-Madison/5_August_2009 5] [http://2009.igem.org/Wisconsin-Madison/6_August_2009 6] [http://2009.igem.org/Wisconsin-Madison/7_August_2009 7] [http://2009.igem.org/Wisconsin-Madison/8_August_2009 8] [http://2009.igem.org/Wisconsin-Madison/9_August_2009 9]
[http://2009.igem.org/Wisconsin-Madison/10_August_2009 10] [http://2009.igem.org/Wisconsin-Madison/11_August_2009 11] [http://2009.igem.org/Wisconsin-Madison/12_August_2009 12] [http://2009.igem.org/Wisconsin-Madison/13_August_2009 13] [http://2009.igem.org/Wisconsin-Madison/14_August_2009 14] [http://2009.igem.org/Wisconsin-Madison/15_August_2009 15] [http://2009.igem.org/Wisconsin-Madison/16_August_2009 16]
[http://2009.igem.org/Wisconsin-Madison/17_August_2009 17] [http://2009.igem.org/Wisconsin-Madison/18_August_2009 18] [http://2009.igem.org/Wisconsin-Madison/19_August_2009 19] [http://2009.igem.org/Wisconsin-Madison/20_August_2009 20] [http://2009.igem.org/Wisconsin-Madison/21_August_2009 21] [http://2009.igem.org/Wisconsin-Madison/22_August_2009 22] [http://2009.igem.org/Wisconsin-Madison/23_August_2009 23]
[http://2009.igem.org/Wisconsin-Madison/24_August_2009 24] [http://2009.igem.org/Wisconsin-Madison/25_August_2009 25] [http://2009.igem.org/Wisconsin-Madison/26_August_2009 26] [http://2009.igem.org/Wisconsin-Madison/27_August_2009 27] [http://2009.igem.org/Wisconsin-Madison/28_August_2009 28] [http://2009.igem.org/Wisconsin-Madison/29_August_2009 29] [http://2009.igem.org/Wisconsin-Madison/30_August_2009 30]
[http://2009.igem.org/Wisconsin-Madison/31_August_2009 31]
September
MTWTFSS
  [http://2009.igem.org/Wisconsin-Madison/1_September_2009 1] [http://2009.igem.org/Wisconsin-Madison/2_September_2009 2] [http://2009.igem.org/Wisconsin-Madison/3_September_2009 3] [http://2009.igem.org/Wisconsin-Madison/4_September_2009 4] [http://2009.igem.org/Wisconsin-Madison/5_September_2009 5] [http://2009.igem.org/Wisconsin-Madison/6_September_2009 6]
[http://2009.igem.org/Wisconsin-Madison/7_September_2009 7] [http://2009.igem.org/Wisconsin-Madison/8_September_2009 8] [http://2009.igem.org/Wisconsin-Madison/9_September_2009 9] [http://2009.igem.org/Wisconsin-Madison/10_September_2009 10] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/11_September_2009&action=edit 11] [http://2009.igem.org/Wisconsin-Madison/12_September_2009 12] [http://2009.igem.org/Wisconsin-Madison/13_September_2009 13]
[http://2009.igem.org/Wisconsin-Madison/14_September_2009 14] [http://2009.igem.org/Wisconsin-Madison/15_September_2009 15] [http://2009.igem.org/Wisconsin-Madison/16_September_2009 16] [http://2009.igem.org/Wisconsin-Madison/17_September_2009 17] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/18_September_2009&action=edit 18] [http://2009.igem.org/Wisconsin-Madison/19_September_2009 19] [http://2009.igem.org/Wisconsin-Madison/20_September_2009 20]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/21_September_2009&action=edit 21] [http://2009.igem.org/Wisconsin-Madison/22_September_2009 22] [http://2009.igem.org/Wisconsin-Madison/23_September_2009 23] [http://2009.igem.org/Wisconsin-Madison/24_September_2009 24] [http://2009.igem.org/Wisconsin-Madison/25_September_2009 25] [http://2009.igem.org/Wisconsin-Madison/26_September_2009 26] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/27_September_2009&action=edit 27]
[http://2009.igem.org/Wisconsin-Madison/28_September_2009 28] [http://2009.igem.org/Wisconsin-Madison/29_September_2009 29] [http://2009.igem.org/Wisconsin-Madison/30_September_2009 30]
October
MTWTFSS
      [http://2009.igem.org/Wisconsin-Madison/1_October_2009 1] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/2_October_2009&action=edit 2] [http://2009.igem.org/Wisconsin-Madison/3_October_2009 3] [http://2009.igem.org/Wisconsin-Madison/4_October_2009 4]
[http://2009.igem.org/Wisconsin-Madison/5_October_2009 5] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/6_October_2009&action=edit 6] [http://2009.igem.org/Wisconsin-Madison/7_October_2009 7] [http://2009.igem.org/Wisconsin-Madison/8_October_2009 8] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/9_October_2009&action=edit 9] [http://2009.igem.org/Wisconsin-Madison/10_October_2009 10] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/11_October_2009&action=edit 11]
[http://2009.igem.org/Wisconsin-Madison/12_October_2009 12] [http://2009.igem.org/Wisconsin-Madison/13_October_2009 13] [http://2009.igem.org/Wisconsin-Madison/14_October_2009 14] [http://2009.igem.org/Wisconsin-Madison/15_October_2009 15] [http://2009.igem.org/Wisconsin-Madison/16_October_2009 16] [http://2009.igem.org/Wisconsin-Madison/17_October_2009 17] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/18_October_2009&action=edit 18]
[http://2009.igem.org/Wisconsin-Madison/19_October_2009 19] [http://2009.igem.org/Wisconsin-Madison/20_October_2009 20] [http://2009.igem.org/Wisconsin-Madison/21_October_2009 21] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/22_October_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/23_October_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/24_October_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/25_October_2009&action=edit 25]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/26_October_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/27_October_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/28_October_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/29_October_2009&action=edit 29] [http://2009.igem.org/Wisconsin-Madison/30_October_2009 30] [http://2009.igem.org/Wisconsin-Madison/31_October_2009 31]

June 11, 2009

Other: Meeting with Brian (Notes – iGEM: 0 Summer Meeting)


Ex 10: Made Terrific Broth for triple transformed cell medium

Inoculation of triple transformation

Induction: 1. Irrabinose - 0.8 – 1% g/100mL

2. Wait 30 minutes

3. 1mM conc. IPTG (100uL/100mL)

4. Layer of Dodecane to trap isopentenol


Ex 11: Salt tolerance of E.coli DH10B:

Use plate reading machine to chart OD over time

ExA: NaCl 0-1 mol in one tenth intervals (NaCl - Mm 58.44) in Liquid salt-free LB

30uL of DH10B ecoli, 200uL of LB

OD taken every 30 min for 48 hours


Conc/NaCl (g)/Actual (g)

0.0M/0.0/0.0

0.1M/ 0.116886/ 0.1157

0.2M/ 0.233772/ 0.2355

0.3M/ 0.350658/ 0.3560

0.4M/ 0.467544/ 0.4633

0.5M/ 0.584430/ 0.5859

0.6M/ 0.701316/ 0.7034

0.7M/ 0.818202/ 0.8156

0.8M/ 0.935088/ 0.9348

0.9M/ 1.051974/ 1.0577

1.0M/ 1.16886/ 1.1692


Inoculation of DH10B to run experiment tomorrow


Ex 9: Transformation of spinach cDNA into E.Coli DH10B

Add 50uL of to ~100ug precipitated DNA from Mike Ziemak

(pREP3-A/spinach PEAMT DNA)


Ex 13 Design: Thermocycles for Choline, Pro U, YhfR, NudF


- inoculated cultures of pSB1A3, pRL1383A, and pMMBRraA in LB media with appropriate antibiotics to remake freezer stocks - NP

- inoculated liquid culture of pNudF, pMBI, pMevT transformed e coli in LB with appropriate antibiotics to test isopentenol production by induction tomorrow - NP

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