Wisconsin-Madison/17 September 2009
From 2009.igem.org
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September 17, 2009
GSMT+BB, GSMT+33, ProU+RFP sequencing
.5 uL DNA (100 ng)
1 uL Big Dye
1.5uL Big Dye Buffer
.25uL Primer (fwd or rev)
5.75uL H2O
Sequencing clean-up using magnetic beads
Confirm sequencing results that GSMT+BB, GSMT+33 and ProU+RFP worked.
rearanged plasmids in freezer
Plasmid Isolation of pBAD35
Cyano Talk with Daniel
Wrote abstract and sent
Inoculation:
- pHawaii (strep)
- pBAD35 (kan)
Clean and Ran Digestion Product
Digestion and Ligation
- SDMT:pBAD18