Team:Imperial College London/Wetlab/Protocols/Cellculture

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=Cell culture protocols=

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! Section
! Assay
! Overview and Aims

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| <b>CC1</b>: Miniprep
| [[Team:Imperial_College_London/ |Link]]
|* Harvest modest amounts of genomic DNA<br>

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| <b>CC2</b>: Cellulase
| [[Team:Imperial_College_London/ |Link]]
|*Harvest large amount of genomic DNA<br>

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| <b>CC3</b>: Cell competence
| [[Team:Imperial_College_London/ |Link]]
| *Making cells more competent to taking up DNA <br>

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| <b>CC4</b>: Ligation
| [[Team:Imperial_College_London/ |Link]]
| *Ligation of different DNA constructs together<<br>

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| <b>CC5</b>: Transformation (BL21)
| [[Team:Imperial_College_London/ |Link]]
| *Transformation into BL21 cells using electrical shock <<br>

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| <b>CC6</b>: Transformation (Top10)
| [[Team:Imperial_College_London/Wetlab/Protocols/Cellculture/TransTop10 |Transformation into Top10]]
| *Transformation into chemically competent Top10 cells <<br>

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| <b>CC7</b>: Make LB plates
| [[Team:Imperial_College_London/Wetlab/Protocols/Cellculture/LBPlates |Make LB plates]]
| *Making plates of LB agar for cell culturing<br>

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| <b>CC7</b>: Extract DNA from registry
| [[Team:Imperial_College_London/Wetlab/Protocols/ExtractingDNA |Extracting DNA]]
| *Extracting the required DNA from the registry<br>
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