Team:Brown/Notebook Meetings/5-27-09

{{Brown}}







'''Brown iGEM 2009'''

'''Phone Conference'''

'''5/27/09'''

'''(Pacific Time)'''

*8:20PM: Meeting starts after a lot of technical difficulties

*8:22: Brad Lowell at Harvard does not have UCP2, Invitrogen may have it looked up UCPs on invitrogen
**What expression vectors has the past iGEM teams used?
**We need E. coli expression vectors 

*8:32	Ahmad, ask them for the UCP

*8:29: Indu wrote a nicely worded email.

*8:31: Picking holins (there’s 3000!)
**There are some in the registry
**UC Berkeley from last year used Holin
**Ahmad will ask them for a couple Holins

*8:49: 	CCDB – stops cell growth – can be another redundant mechanism
**It is in the registry

*8:56: All of the parts so far are in the registry, we need a unique part to submit to the registry

*9:01: We need to schedule a meeting with the panel for when we get back!
**June 17th
**Email the professors
**It would be nice to have Gary Wessel email them.

*9:08: Michael write an email out to the professors inviting them
**Have the draft proofread by the group
**Have Wessel forward it to everybody

*9:15: Somebody else – research a tight repressor!!

*9:17: Indu will follow up phone call the lab for the tick protein
**Second idea to the histamine binding protein – have something that also binds IgE antibodies
**This provides redundancy – both binding current histamine and stopping further secretion of histamine
**Link them with glycine bridge

*9:26: Probiotic system: natural bacteria that secretes protein into bloodstream
**We have getting it into the system from MIT yogurt
**We have intestinal implantation BactoKidney
**How do we get the bifunctional protein secreted?

*9:44: There is a lady named Edith that Will will email – she can help us on probiotic drug transport across organs
**Ashley, Eli, Will, Minoo can research probiotic 	

*9:45: Indu found a paper on protein that binds IGE antibody – she will put it on the google group
**Somebody needs to research IGE
**Eli will put up a paper about packaging
**Will is emailing about drug delivery systems

*9:46: Any paper that may be useful to either project will be put on the google groups

*9:56: Before 8pm PST – everyone go on the google group and build on the research that’s already been done.

*10:00: Secretion: Eli, Will, Ashley
**OK everyone just decide what to research on the google group.  

*10:01: We are meeting on SUNDAY MAY 31 SAME TIME (Monday for Minoo)

*10:02: Put up minutes.

*10:16: bye everybody