Team:VictoriaBC/Labnotebook

Current Parts

 
Assembly Parts

 

 

 

The first time a part shows up in a gel it is bold.

 

Preparing buffers

[[Lab June 25 2009]]

 

Making plates

[[Lab June 29 2009]]

Starting to make Qiagen reagents

[[Lab June 30 2009]]

pH'ing Tris and other stocks

[[Lab July 2 2009]]

More Qiagen work

[[Lab July 3 2009]]

 

Test Quiagen Miniprep and gel

[[Lab July 6 2009]]

Prepare CCMB80 buffer

[[Lab July 10 2009]]

 

Make SOB medium

[[Lab July 13 2009]]

Checking on failed DH5alpha plates

[[Lab July 14 2009]] 

Diagnosing contamination problems

[[Lab July 15 2009]]

New DH5alpha plates

[[Lab July 17 2009]]

 

Acheived DH5α growth

[[Lab July 20 2009]]

Make competent cells following top-10 protocol

[[Lab July 21 2009]]

Measurement of competence

[[Lab July 22 2009]]

Failed competence, started on BCMB301 competent cells protocol

[[Lab July 23 2009]]

Test of competence on new cells, greater quantity of pUC18

[[Lab July 24 2009]]

 

Successful test of competence with pUC18

[[Lab July 27 2009]]

Initial messing with rehydration protocol

[[Lab July 28 2009]]

Successful biobrick transformation

[[Lab July 29 2009]]

Nandrop rehydrations, more transformations

[[Lab July 30 2009]] 

Failed lambda cI  promoter

[[Lab July 31 2009]]

 

Checked weekend culture of RFP

[[Lab Aug 4 2009]]

Another nanodrop, more rehydrations and transformations, ran a gel (B0015 Amp/Kan, J06504 Amp, R0051 Amp, B0034 Amp)

[[Lab Aug 5 2009]]

More transformations, struggling with I0500

[[Lab Aug 6 2009]]

Struggling with Kan growth, more nanodrop rehydrations

[[Lab Aug 7 2009]]

 

Tried to induce high copy for I0500 using IPTG

[[Lab Aug 10 2009]]

Make ccdB competent cells, miniprep plasmids, more transformations

[[Lab Aug 11 2009]]

Try again at making ccdb strains with Kan and Cm resistances, more transformations

[[Lab Aug 12 2009]]

Check on ccdB strains, minipreps, etc.

[[Lab Aug 13 2009]]

Make new ccdB competent cells, check on cultures, miniprep, gels (P1010 Kan, J23102, J45120, J45200, K145303, P0151, I0500, C0083, E0422, R1051)

[[Lab Aug 14 2009]]

 

Brainstorming, trying to transform P1010

[[Lab Aug 17 2009]] 

Possible success in transforming P1010

[[Lab Aug 18 2009]] 

Miniprep and check plasmids, cut and ligated and transformed first assemblies, a few broth cultures, gel (P1010 Kan, P1010 Cm C)

[[Lab Aug 19 2009]]

Transformed second assemblies, gel (digests)

[[Lab Aug 20 2009]]

Gels (K235000, K235001 and K235002)

[[Lab Aug 21 2009]]

 

Gel (linearized DNA)

[[Lab Aug 24 2009]]

Gels and assemblies (K145303 better)

[[Lab Aug 25 2009]]

Pour Cm plates

[[Lab Aug 26 2009]] 

Re-plate ligation products, new competent cells, failed 2% gels (digests)

[[Lab Aug 27 2009]] 

Re-plate remaining assemblies, start fridge and freezer cleanup, gels (digests)

[[Lab Aug 28 2009]] 

Brainstorming

[[Lab Aug 29, 2009]]

 

 

Plan for the week, gels (?)

[[Lab Aug 31 2009]] 

Check P1010Kan, broth culture, gel (K081005, K098988)

[[Lab Sept 01 2009]]

Reassembling K235003, 05 and 10, starting J04450, plating stabs, talking to many people

[[Lab Sept 02 2009]]

Broth cultures

[[Lab Sept 3 2009]]

Lab move, miniprep and gels (K235003 Cm, K235005 Cm, K235010 Cm, J04450 Kan (+IPTG), J04450 Kan (-IPTG), P1010 Kan, P1010 Amp, R0051 Amp, I0500 Kan (+IPTG), I0500 Kan (-IPTG))

[[Lab Sep 4 2009]]

 

Scheduling a lab meetup for Wednesday, some assemblies, a subculture

[[Lab Sept 08 2009]]

Talk-talk and resources

[[Lab Sep 9 2009]] 

Transformations

[[Lab Sep 10 2009]]

Broth cultures and rehydrations

[[Lab Sep 11 2009]]

Minipreps and broth cultures

[[Lab Sep 12 2009]]

 

Aborted miniprep

[[Lab Sep 14 2009]]

Broth cultures

[[Lab Sep 15 2009]]

Minipreps

[[Lab Sep 16 2009]]

Gels (K235009, C0012, K235013, K115017, K235014, E0240, K235016, K235017, R0051)

[[Lab Sep 17 2009]]

Flourescence microscopy - failed

[[Lab Sep 18 2009]]

 

Assemblies, transformations

[[Lab Sep 22 2009]]

Broth cultures not done

[[Lab Sep 23 2009]]

Broth cultures

[[Lab Sep 24 2009]]

Miniprep, gels (failed)

[[Lab Sep 25 2009]]

Subcultures

[[Lab Sep 27 2009]]

 

Check cultures

[[Lab Sep 28 2009]]

 

Transformations

[[Lab Oct 8 2009]]

Broth cultures and transformations

[[Lab Oct 9 2009]]

Minipreps and gel (K235019 Amp, K235020 Amp, K235021 Amp, K115017 Amp, C0012 Amp, J04630 Amp, J23070 Amp, R0051 Amp)

[[Lab Oct 10 2009]]

Minipreps

[[Lab Oct 11 2009]]

 

Broth cultures

[[Lab Oct 12 2009]]

Minipreps, gel (K235030 Kan, K235030 Chl, K235031 Chl, E0840 Amp, and K115020 Amp)

[[Lab Oct 13 2009]]

Broth cultures

[[Lab Oct 14 2009]]

Flourescence microscopy, gel (K235032 Kan, K235033 Kan, K235036 Kan, K235037 Chl)

[[Lab Oct 15 2009]]

Flourescence microscopy,assemblies, broth cultures

[[Lab Oct 16 2009]]