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Team:VictoriaBC/Labnotebook
From 2009.igem.org
Current Parts
Assembly Parts
The first time a part shows up in a gel it is bold.
Preparing buffers
Making plates
Starting to make Qiagen reagents
pH'ing Tris and other stocks
More Qiagen work
Test Quiagen Miniprep and gel
Prepare CCMB80 buffer
Make SOB medium
Checking on failed DH5alpha plates
Diagnosing contamination problems
New DH5alpha plates
Acheived DH5α growth
Make competent cells following top-10 protocol
Measurement of competence
Failed competence, started on BCMB301 competent cells protocol
Test of competence on new cells, greater quantity of pUC18
Successful test of competence with pUC18
Initial messing with rehydration protocol
Successful biobrick transformation
Nandrop rehydrations, more transformations
Failed lambda cI promoter
Checked weekend culture of RFP
Another nanodrop, more rehydrations and transformations, ran a gel (B0015 Amp/Kan, J06504 Amp, R0051 Amp, B0034 Amp)
More transformations, struggling with I0500
Struggling with Kan growth, more nanodrop rehydrations
Tried to induce high copy for I0500 using IPTG
Make ccdB competent cells, miniprep plasmids, more transformations
Try again at making ccdb strains with Kan and Cm resistances, more transformations
Check on ccdB strains, minipreps, etc.
Make new ccdB competent cells, check on cultures, miniprep, gels (P1010 Kan, J23102, J45120, J45200, K145303, P0151, I0500, C0083, E0422, R1051)
Brainstorming, trying to transform P1010
Possible success in transforming P1010
Miniprep and check plasmids, cut and ligated and transformed first assemblies, a few broth cultures, gel (P1010 Kan, P1010 Cm C)
Transformed second assemblies, gel (digests)
Gels (K235000, K235001 and K235002)
Gel (linearized DNA)
Gels and assemblies (K145303 better)
Pour Cm plates
Re-plate ligation products, new competent cells, failed 2% gels (digests)
Re-plate remaining assemblies, start fridge and freezer cleanup, gels (digests)
Brainstorming
Plan for the week, gels (?)
Check P1010Kan, broth culture, gel (K081005, K098988)
Reassembling K235003, 05 and 10, starting J04450, plating stabs, talking to many people
Broth cultures
Lab move, miniprep and gels (K235003 Cm, K235005 Cm, K235010 Cm, J04450 Kan (+IPTG), J04450 Kan (-IPTG), P1010 Kan, P1010 Amp, R0051 Amp, I0500 Kan (+IPTG), I0500 Kan (-IPTG))
Scheduling a lab meetup for Wednesday, some assemblies, a subculture
Talk-talk and resources
Transformations
Broth cultures and rehydrations
Minipreps and broth cultures
Aborted miniprep
Broth cultures
Minipreps
Gels (K235009, C0012, K235013, K115017, K235014, E0240, K235016, K235017, R0051)
Flourescence microscopy - failed
Assemblies, transformations
Broth cultures not done
Broth cultures
Miniprep, gels (failed)
Subcultures
Check cultures
Transformations
Broth cultures and transformations
Minipreps and gel (K235019 Amp, K235020 Amp, K235021 Amp, K115017 Amp, C0012 Amp, J04630 Amp, J23070 Amp, R0051 Amp)
Minipreps
Broth cultures
Minipreps, gel (K235030 Kan, K235030 Chl, K235031 Chl, E0840 Amp, and K115020 Amp)
Broth cultures
Flourescence microscopy, gel (K235032 Kan, K235033 Kan, K235036 Kan, K235037 Chl)
Flourescence microscopy,assemblies, broth cultures
