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Virginia Commonwealth/15 July 2009
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| + | |align="center" width="150pt"|{{#calendar: title=Virginia_Commonwealth |year=2009 | month=05}} | ||
| + | |align="center" width="150pt"|{{#calendar: title=Virginia_Commonwealth |year=2009 | month=06}} | ||
| + | |align="center" width="150pt"|{{#calendar: title=Virginia_Commonwealth |year=2009 | month=07}} | ||
| + | |align="center" width="150pt"|{{#calendar: title=Virginia_Commonwealth |year=2009 | month=08}} | ||
| + | |align="center" width="150pt"|{{#calendar: title=Virginia_Commonwealth |year=2009 | month=09}} | ||
| + | |align="center" width="150pt"|{{#calendar: title=Virginia_Commonwealth |year=2009 | month=10}} | ||
| + | |} | ||
| + | ==Wednesday 15 July 2009== | ||
===Results=== | ===Results=== | ||
* Results of Retransformation with pSB4C5 backbone in NEB 10 beta | * Results of Retransformation with pSB4C5 backbone in NEB 10 beta | ||
| - | {| cellpadding=" | + | {| cellpadding="5" border="1" padding="5" |
|+ | |+ | ||
|- | |- | ||
| - | ! Promoter!! Reporter !! Resistance/Plate !! Growth Observation | + | ! Promoter!! Reporter !! Resistance/Plate !! Growth Observation |
|- | |- | ||
! J23101 | ! J23101 | ||
| - | | E0240 || Cm || | + | | E0240 || Cm || n/a |
|- | |- | ||
! J23101 | ! J23101 | ||
| - | | J06702 || Cm || | + | | J06702 || Cm || n/a |
|- | |- | ||
! J23102 | ! J23102 | ||
| - | | E0240 || Cm || | + | | E0240 || Cm || n/a |
|- | |- | ||
! J23102 | ! J23102 | ||
| - | | J06702 || Cm || | + | | J06702 || Cm || n/a |
|- | |- | ||
! J23103 | ! J23103 | ||
| - | | E0240 || Cm || | + | | E0240 || Cm || n/a |
|- | |- | ||
! J23103 | ! J23103 | ||
| - | | J06702 || Cm || | + | | J06702 || Cm || n/a |
|- | |- | ||
! J23104 | ! J23104 | ||
| - | | E0240 || Cm || | + | | E0240 || Cm || n/a |
|- | |- | ||
! J23104 | ! J23104 | ||
| - | | J06702 || Cm || | + | | J06702 || Cm || n/a |
|- | |- | ||
! J23105 | ! J23105 | ||
| - | | E0240 || Cm || | + | | E0240 || Cm || n/a |
|- | |- | ||
! J23105 | ! J23105 | ||
| - | | J06702 || Cm || | + | | J06702 || Cm || n/a |
|- | |- | ||
! J23107 | ! J23107 | ||
| - | | E0240 || Cm || | + | | E0240 || Cm || n/a |
|- | |- | ||
! J23107 | ! J23107 | ||
| - | | J06702 || Cm || | + | | J06702 || Cm || n/a |
|} | |} | ||
*Controls with NEB 10 beta | *Controls with NEB 10 beta | ||
| - | {| cellpadding=" | + | {| cellpadding="5" border="1" padding="5" |
|+ | |+ | ||
|- | |- | ||
| - | ! Control !! Resistance !! Plate !! Growth Observation | + | ! Control !! Resistance !! Plate !! Growth Observation |
|- | |- | ||
! Positive (+) | ! Positive (+) | ||
| - | | none || LB || lawn | + | | none || LB || lawn |
|- | |- | ||
! Electroshocked (+) | ! Electroshocked (+) | ||
| - | | none || LB || lawn | + | | none || LB || lawn |
|- | |- | ||
! Negative (-) | ! Negative (-) | ||
| - | | none || Cm || no cell growth | + | | none || Cm || no cell growth |
|} | |} | ||
[[User:Trentay|Trentay]] 16:59, 15 July 2009 (UTC) | [[User:Trentay|Trentay]] 16:59, 15 July 2009 (UTC) | ||
| + | |||
| + | ''Craig and Clay'' | ||
| + | * The ampicillin plates again showed no growth. However, the non-selective plates displayed lawn growth. | ||
---- | ---- | ||
| Line 77: | Line 118: | ||
* Make cell stock of Biobrick part: J23106, J06702, pSB4C5 | * Make cell stock of Biobrick part: J23106, J06702, pSB4C5 | ||
**2mL of BioBrick overnight culture was diluted in 4mL of LB broth | **2mL of BioBrick overnight culture was diluted in 4mL of LB broth | ||
| + | * Organize Notebook(s) | ||
[[User:Trentay|Trentay]] 16:59, 15 July 2009 (UTC) | [[User:Trentay|Trentay]] 16:59, 15 July 2009 (UTC) | ||
| + | |||
| + | ''Craig and Clay'' | ||
| + | * The cells growing on the non-selective media will be streaked onto plates containing kanamycin and plates containing chloramphenicol. | ||
---- | ---- | ||
| Line 84: | Line 129: | ||
* All LB was prepared and autoclaved | * All LB was prepared and autoclaved | ||
* Overnight culture dilutions were completed | * Overnight culture dilutions were completed | ||
| - | [[User:Trentay|Trentay]] | + | * Electrocompetency of NEB 10 beta was completed |
| + | * LB and Sterile water was made | ||
| + | * 10% glycerol stock was made | ||
| + | [[User:Trentay|Trentay]] 14:13, 16 July 2009 (UTC) | ||
| + | |||
| + | ''Kevin & Adam'' | ||
| + | *run gel on digestion from yesterday assembly. | ||
| + | |||
| + | ''Craig and Clay'' | ||
| + | * Cells possibly containing the limonene synthesis related parts grown on the plates containing no antibiotics were taken and plated onto kanamycin-selective and chloramphenicol-selective plates. | ||
Latest revision as of 20:49, 17 October 2009
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Contents |
Wednesday 15 July 2009
Results
- Results of Retransformation with pSB4C5 backbone in NEB 10 beta
| Promoter | Reporter | Resistance/Plate | Growth Observation |
|---|---|---|---|
| J23101 | E0240 | Cm | n/a |
| J23101 | J06702 | Cm | n/a |
| J23102 | E0240 | Cm | n/a |
| J23102 | J06702 | Cm | n/a |
| J23103 | E0240 | Cm | n/a |
| J23103 | J06702 | Cm | n/a |
| J23104 | E0240 | Cm | n/a |
| J23104 | J06702 | Cm | n/a |
| J23105 | E0240 | Cm | n/a |
| J23105 | J06702 | Cm | n/a |
| J23107 | E0240 | Cm | n/a |
| J23107 | J06702 | Cm | n/a |
- Controls with NEB 10 beta
| Control | Resistance | Plate | Growth Observation |
|---|---|---|---|
| Positive (+) | none | LB | lawn |
| Electroshocked (+) | none | LB | lawn |
| Negative (-) | none | Cm | no cell growth |
Trentay 16:59, 15 July 2009 (UTC)
Craig and Clay
- The ampicillin plates again showed no growth. However, the non-selective plates displayed lawn growth.
Tasks
Maria and Afton
- Make more electrocompetent NEB 10 beta
- 500 mL LB autoclaved in 1 L flask
- 1 L LB autoclaved
- 2mL of NEB 10 beta overnight culture was diluted in 4mL of LB broth
- Two bottles of LB Agar were prepared
- for Amp and Cm plates
- Three bottles of LB prepared
- two liquid LB+Amp will be prepared
- one liquid LB+Cm will be prepared
- Let transformed BioBrick parts continue to grow overnight (on plates) before colonies are picked
- Make cell stock of Biobrick part: J23106, J06702, pSB4C5
- 2mL of BioBrick overnight culture was diluted in 4mL of LB broth
- Organize Notebook(s)
Trentay 16:59, 15 July 2009 (UTC)
Craig and Clay
- The cells growing on the non-selective media will be streaked onto plates containing kanamycin and plates containing chloramphenicol.
Wetlab
Maria and Afton
- All LB was prepared and autoclaved
- Overnight culture dilutions were completed
- Electrocompetency of NEB 10 beta was completed
- LB and Sterile water was made
- 10% glycerol stock was made
Trentay 14:13, 16 July 2009 (UTC)
Kevin & Adam
- run gel on digestion from yesterday assembly.
Craig and Clay
- Cells possibly containing the limonene synthesis related parts grown on the plates containing no antibiotics were taken and plated onto kanamycin-selective and chloramphenicol-selective plates.
