"
Team:Wisconsin-Madison/Restriction Digestion
From 2009.igem.org
(Difference between revisions)
(New page: {{Wisconsin-Madison Template}} {{Wisconsin-Madison Note Template}} <br> {| class="wikitable" border="0" align="center" width=790px |style="text-align:left" style="vertical-| <center> ''...) |
|||
| (One intermediate revision not shown) | |||
| Line 9: | Line 9: | ||
</center> | </center> | ||
| - | Use smallest micro cuvettes and mix: | + | Use smallest micro cuvettes and mix (total volume 18 μL): |
| - | + | * 1.8 μL Buffer (10x) | |
| - | + | * 0.18 μL BSA +3 (100x) | |
| - | + | * 12 μL MiliQ H20 | |
| - | + | * 3 μL DNA | |
| - | + | * 1 μL Enzyme (100x) | |
| - | Incubate in water bath at 37 C for 2 hours | + | |
| - | + | Incubate in water bath at 37 C for 2 hours: | |
| + | * 3.6 μL Dye (6x) | ||
<center>[[Team:Wisconsin-Madison/Notebook_Protocols|'''Back to Protocols''']]</center> | <center>[[Team:Wisconsin-Madison/Notebook_Protocols|'''Back to Protocols''']]</center> | ||
Latest revision as of 22:17, 17 October 2009
Restriction Enzyme Mapping Use smallest micro cuvettes and mix (total volume 18 μL):
Incubate in water bath at 37 C for 2 hours:
|
