Team:Wisconsin-Madison/Cloning Protocols

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Revision as of 23:30, 17 October 2009



Overview of Cloning Protocol


  • Solid Culture
  • Liquid Culture
  • Plasmid Isolation
  • Restriction Enzyme Mapping for Verification
  • PCR to add BioBrick Restriction Cut Sites (Primer Design)
  • PCR Plasmid Cleanup
  • Concentration Verification
  • Digestion
  • Digestion Clean Up
  • Concentration Verification
  • Ligation
  • Transformation
  • Colony PCR Screening


Procedures for these can be found in Protocol Section
Back to Protocols
Retrieved from "http://2009.igem.org/Team:Wisconsin-Madison/Cloning_Protocols"