Template:UNIPV-Pavia/Protocols
From 2009.igem.org
(Difference between revisions)
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*[[Team:UNIPV-Pavia/Methods_Materials/LB|LB medium]] | *[[Team:UNIPV-Pavia/Methods_Materials/LB|LB medium]] | ||
*[[Team:UNIPV-Pavia/Methods_Materials/Resuspension|DNA resuspension from iGEM 2009 plates]] | *[[Team:UNIPV-Pavia/Methods_Materials/Resuspension|DNA resuspension from iGEM 2009 plates]] | ||
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*[[Team:UNIPV-Pavia/Methods_Materials/Ligation|Ligation]] | *[[Team:UNIPV-Pavia/Methods_Materials/Ligation|Ligation]] | ||
*[[Team:UNIPV-Pavia/Methods_Materials/PCR|PCR]] | *[[Team:UNIPV-Pavia/Methods_Materials/PCR|PCR]] | ||
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Revision as of 17:04, 22 June 2009
- LB medium
- DNA resuspension from iGEM 2009 plates
- Transformation
- X-Gal staining protocol for beta galactosidase (blue/white screening)
- Glycerol stocks
- Plasmid extraction
- BioBrick digestion with restriction enzymes
- DNA gel extraction
- DNA precipitation with sodium acetate
- Ligation
- PCR