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Template:UNIPV-Pavia/Protocols
From 2009.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | *[[Team:UNIPV-Pavia/Methods_Materials/LB|LB medium]] | + | *[[Team:UNIPV-Pavia/Methods_Materials/LB#LB medium|LB medium]] |
*[[Team:UNIPV-Pavia/Methods_Materials/Resuspension|DNA resuspension from iGEM 2009 plates]] | *[[Team:UNIPV-Pavia/Methods_Materials/Resuspension|DNA resuspension from iGEM 2009 plates]] | ||
*[[Team:UNIPV-Pavia/Methods_Materials/Transformation|Transformation]] | *[[Team:UNIPV-Pavia/Methods_Materials/Transformation|Transformation]] | ||
| Line 6: | Line 6: | ||
*[[Team:UNIPV-Pavia/Methods_Materials/PlasmidExtraction|Plasmid extraction]] | *[[Team:UNIPV-Pavia/Methods_Materials/PlasmidExtraction|Plasmid extraction]] | ||
*[[Team:UNIPV-Pavia/Methods_Materials/Digestion|BioBrick digestion with restriction enzymes]] | *[[Team:UNIPV-Pavia/Methods_Materials/Digestion|BioBrick digestion with restriction enzymes]] | ||
| + | *[[Team:UNIPV-Pavia/Methods_Materials/Electrophoresis|Electrophoresis]] | ||
*[[Team:UNIPV-Pavia/Methods_Materials/GelExtraction|DNA gel extraction]] | *[[Team:UNIPV-Pavia/Methods_Materials/GelExtraction|DNA gel extraction]] | ||
*[[Team:UNIPV-Pavia/Protocols/Precipitation|DNA precipitation with sodium acetate]] | *[[Team:UNIPV-Pavia/Protocols/Precipitation|DNA precipitation with sodium acetate]] | ||
*[[Team:UNIPV-Pavia/Methods_Materials/Ligation|Ligation]] | *[[Team:UNIPV-Pavia/Methods_Materials/Ligation|Ligation]] | ||
*[[Team:UNIPV-Pavia/Methods_Materials/PCR|PCR]] | *[[Team:UNIPV-Pavia/Methods_Materials/PCR|PCR]] | ||
Revision as of 17:12, 22 June 2009
- LB medium
- DNA resuspension from iGEM 2009 plates
- Transformation
- X-Gal staining protocol for beta galactosidase (blue/white screening)
- Glycerol stocks
- Plasmid extraction
- BioBrick digestion with restriction enzymes
- Electrophoresis
- DNA gel extraction
- DNA precipitation with sodium acetate
- Ligation
- PCR
