Team:UNIPV-Pavia/Methods Materials/Resuspension

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(New page: {{UNIPV-Pavia/Protocols}} <br> == '''DNA resuspension from iGEM 2009 plates''' == ''(Estimated time: 30 min)'' <br> <br> '''Materials needed:''' *'''iGEM 2009 plates''' *'''PC with intern...)
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== '''LB medium''' ==
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''(Estimated time: 10 min + 4 hours of autoclavation and cooling)''
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<br>
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<br>
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'''Materials needed:'''
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*'''NaCl'''
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*'''BactoTryptone'''
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*'''Yeast extract '''
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*'''ddH2O'''
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*'''Agar'''
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*'''Clean bottle or E-flask'''
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<br>
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*For a final volume of 1 l, put:
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**10 g NaCl
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**10 g BactoTryptone
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**5 g Yeast extract
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**15 g Agar (only for LB plates)
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*into 1 l of ddH2O.
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*Autoclave the solution.
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*Let it cool to ~40-45°C
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*Add the proper antibiotic if needed.
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*ONLY FOR PLATES:
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**Pour an homogenous layer of agar LB into Petri plates under the hood.
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**Let agar LB polymerase.
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*Cover and store at +4°C.
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*Always check for contaminations before using!
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<br>
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== '''DNA resuspension from iGEM 2009 plates''' ==  
== '''DNA resuspension from iGEM 2009 plates''' ==  

Revision as of 17:17, 22 June 2009

EthanolPVanimation.gif



Protocols



LB medium

(Estimated time: 10 min + 4 hours of autoclavation and cooling)

Materials needed:

  • NaCl
  • BactoTryptone
  • Yeast extract
  • ddH2O
  • Agar
  • Clean bottle or E-flask


  • For a final volume of 1 l, put:
    • 10 g NaCl
    • 10 g BactoTryptone
    • 5 g Yeast extract
    • 15 g Agar (only for LB plates)
  • into 1 l of ddH2O.
  • Autoclave the solution.
  • Let it cool to ~40-45°C
  • Add the proper antibiotic if needed.
  • ONLY FOR PLATES:
    • Pour an homogenous layer of agar LB into Petri plates under the hood.
    • Let agar LB polymerase.
  • Cover and store at +4°C.
  • Always check for contaminations before using!



DNA resuspension from iGEM 2009 plates

(Estimated time: 30 min)

Materials needed:

  • iGEM 2009 plates
  • PC with internet connection :-)
  • ddH20
  • 0.5 ml sterile Eppendorf tubes


  • Find the correct position of the desired DNA from the iGEM 2009 plates
  • Resuspend with ddH20 and transfer in an Eppendorf tubes
  • Store at -20°C
Retrieved from "http://2009.igem.org/Team:UNIPV-Pavia/Methods_Materials/Resuspension"