Team:UNIPV-Pavia/Methods Materials/PlasmidExtraction

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    <font face="Pristina" size="50" ><b>Protocols</b></font>
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<h1>Plasmid extraction</h1>
<h1>Plasmid extraction</h1>
''(Estimated time: 3 hours if you use QIAGEN Plasmid Mini Kit; 1 hour if you use QIAGEN QIAprep Spin Miniprep Kit)''
''(Estimated time: 3 hours if you use QIAGEN Plasmid Mini Kit; 1 hour if you use QIAGEN QIAprep Spin Miniprep Kit)''

Revision as of 17:21, 22 June 2009

EthanolPVanimation.gif



Protocols


Plasmid extraction

(Estimated time: 3 hours if you use QIAGEN Plasmid Mini Kit; 1 hour if you use QIAGEN QIAprep Spin Miniprep Kit)

Materials needed:

  • 5 ml or 9 ml (for higher yields) overnight mini-culture
  • QIAGEN QIAprep Spin Miniprep Kit


  • Follow QIAGEN standard protocol: [http://www1.qiagen.com/literature/render.aspx?id=370&tp=4 QIAGEN QIAprep Spin Miniprep protocol]


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