Team:UNIPV-Pavia/Protocols/Precipitation

From 2009.igem.org

(Difference between revisions)
(New page: <h1>DNA precipitation with sodium acetate</h1> ''(estimated time: 1 hour)'' <br> <br> '''Materials needed:''' *'''sodium acetate''' *'''absolute ethanol''' *'''ethanol 70%''' *'''ddH2O'''...)
Line 1: Line 1:
 +
{{UNIPV-Pavia/StyleCss}}
 +
<html>
 +
<head>
 +
<link rel="stylesheet" type="text/css" href="http://bioinfo.unipv.it/igem/templates/SpryAssets/SpryMenuBar.js" title="Stile"/>
 +
</head>
 +
<body>
 +
<table width="100%">
 +
  <tr>
 +
<td align="center">
 +
<img width="100%" height="180px" src="https://static.igem.org/mediawiki/2009/c/c2/UNIPV_title_MandM.jpg" ></img>
 +
</td>
 +
  </tr>
 +
</table>
 +
</body>
 +
</html>
 +
 +
<table width="100%">
 +
  <tr>
 +
    <td valign="top" width= "23%" rowspan="2">
 +
{{UNIPV-Pavia/Menu}}
 +
    </td>
 +
<html>
 +
    <td width="50%"></td>
 +
    <td align="center"><br><bR>
 +
    <font face="Pristina" size="50" ><b>Protocols</b></font>
 +
    </td>
 +
</html>
 +
  </tr>
 +
<tr>
 +
  <td> {{UNIPV-Pavia/Protocols}}</td>
 +
</tr>
 +
</table>
 +
<h1>DNA precipitation with sodium acetate</h1>
<h1>DNA precipitation with sodium acetate</h1>
''(estimated time: 1 hour)''
''(estimated time: 1 hour)''

Revision as of 17:26, 22 June 2009

EthanolPVanimation.gif



Protocols

DNA precipitation with sodium acetate

(estimated time: 1 hour)

Materials needed:

  • sodium acetate
  • absolute ethanol
  • ethanol 70%
  • ddH2O
  • previously cut plasmid


  • We use it only if the miniprep did not give us a high yield of DNA
  • Add 1/10 digestion volume of sodium acetate 3 M
  • Add 2.5 digestion volume of absolute ethanol
  • Freeze at -80°C for 30 min
  • Centrifuge at 13000 rpm, 4°C for 20 min
  • Decant supernatant
  • Add 50 µl of 70% ethanol
  • Centrifuge at 13000 rpm, 4°C for 20 min
  • Remove all supernatant with a pipette
  • Air dry pellet until ethanol is totally removed
  • Elute with 5 µl ddH2O


Retrieved from "http://2009.igem.org/Team:UNIPV-Pavia/Protocols/Precipitation"