Team:Uppsala-Sweden/Parts

From 2009.igem.org

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     <li class="toclevel-1"><a href="#reg"><span class="tocnumber">1</span> <span class="toctext">Regulatory Elements</span></a>
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     <a class="ah2" name="pro">Promoter</a>
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     <a class="ah2" name="pro">Promoter</h2></a>
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     <a class="ah1" name="prod">Alcohol Production</a>
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     <a class="ah1" name="but">Butanol Parts, Intermediates and Devices</a>
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     <a class="ah2" name="eth1">Ethanol Parts and Devices</a>
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     <a class="ah2" name="eth2">Ethanol Parts, Intermediates and Devices with adh2 from <i>S. cerevisae</i></a>
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     <a class="ah2" name="eth2">Ethanol Parts, Intermediates and Devices with adh2 from <i>S. cerevisae</i></h2></a>
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     <a class="ah1" name="pyr">Pyruvate Level Increasing Approaches</a>
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     <a class="ah2" name="rna">antisense RNA Approach</a>
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Revision as of 17:44, 18 October 2009




Contents


Parts

Here we list all the BioBricks we designed, constructed and used during the project.

Regulatory Elements
Promoter
BBa_K273019 Regulatory pPetE 429

Alcohol Production
Butanol Parts, Intermediates and Devices
BBa_K273006 Coding a-ketoisovalerate decarboxylase (kivd) codon optimized 1650
BBa_K273008 Intermediate RBS - a-ketoisovalerate decarboxylase (kivd) codon optimized 1668
BBa_K273005 Coding Alcohol Dehydrogenase 2 (Yadh2) 1050
BBa_K273007 Intermediate RBS - Alcohol Dehydrogenase 2 (Yadh2) 1068
BBa_K273009 Intermediate RBS - adh2(Y) - Terminator 1205
BBa_K273012 Intermediate RBS - kivd - RBS - adh2 - Ter 2881
BBa_K273011 Composite PLlac - RBS - kivd - RBS - adh2(Y) - Ter 2944
BBa_K273012 Composite pPetE - RBS - kivd - RBS - adh2(Y) - Ter 3318
Ethanol Parts and Devices
BBa_K273000 Coding Pyruvate decarboxylase (pdc) 1710
BBa_K273001 Coding Alcohol dehydrogenase (Zadh2) 1155
BBa_K273004 Intermediate RBS - Pyruvate decarboxylase (pdc) 1728
BBa_K273017 Intermediate RBS - Alcohol dehydrogenase (Zadh2) 1173
BBa_K273018 Intermediate RBS - adh2(Z) - Ter 1310
BBa_K273013 Intermediate RBS - pdc - RBS -adh2(Z) - Ter 3046
BBa_K273056 Composite PLlac - RBS - pdc - RBS - adh2(Z) - Ter 3109
BBa_K273057 Composite pPetE - RBS - pdc - RBS - adh2(Z) - Ter 3483
Ethanol Parts, Intermediates and Devices with adh2 from S. cerevisae
BBa_K273014 Intermediate RBS + pdc + RBS + adh2(Y) + Ter 2941
BBa_K273015 Composite PLlac - RBS - pdc - RBS - adh2(Y) - Ter 3004
BBa_K273016 Composite pPetE - RBS - pdc - RBS - adh2(Y) - Ter 3378

Pyruvate Level Increasing Approaches
Pir Approach
BBa_K273002 Coding Pirin like protein (pirA) 702
BBa_K273003 Coding Pirin like protein (pirB) 240
Antisense RNA Approach
BBa_K273020 RNA E1A1 20
BBa_K273021 RNA E1A2 30
BBa_K273022 RNA E1A3 50
BBa_K273023 RNA E1A4 30
BBa_K273024 RNA E1B1 30
BBa_K273025 RNA E1B2 30
BBa_K273026 RNA E1B3 20
BBa_K273027 RNA E1B4 37
BBa_K273028 RNA E21 30
BBa_K273029 RNA E22 30
BBa_K273030 RNA E23 20
BBa_K273031 RNA E24 50
BBa_K273032 Intermediate E1A1 - Ter 157
BBa_K273033 Intermediate E1A2 - Ter 167
BBa_K273034 Intermediate E1A3 - Ter 187
BBa_K273035 Intermediate E1A4 - Ter 167
BBa_K273036 Intermediate E1B1 - Ter 167
BBa_K273037 Intermediate E1B2 - Ter 167
BBa_K273038 Intermediate E1B3 - Ter 157
BBa_K273039 Intermediate E1B4 - Ter 174
BBa_K273040 Intermediate E21 - Ter 167
BBa_K273041 Intermediate E22 - Ter 167
BBa_K273042 Intermediate E23 - Ter 157
BBa_K273043 Intermediate E24 - Ter 187
BBa_K273044 Composite pPetE - E1A1 - Ter 594
BBa_K273045 Composite pPetE - E1A2 - Ter 604
BBa_K273046 Composite pPetE - E1A3 - Ter 624
BBa_K273047 Composite pPetE - E1A4 - Ter 604
BBa_K273048 Composite pPetE - E1B1 - Ter 604
BBa_K273049 Composite pPetE - E1B2 - Ter 604
BBa_K273050 Composite pPetE - E1B3 - Ter 594
BBa_K273051 Composite pPetE - E1B4 - Ter 611
BBa_K273052 Composite pPetE - E21 - Ter 604
BBa_K273053 Composite pPetE - E22 - Ter 604
BBa_K273054 Composite pPetE - E23 - Ter 594
BBa_K273055 Composite pPetE - E24 - Ter 624






Bioneer Biolegio Clontech Uppsala Genome Center

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