Team:MIT/Projects

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Project 1: Metabolic Engineering of PCB Synthesis in Yeast

Brief Description

Our project uses the PhyB-PIF3 system to create this fast-reversible switch. Phycocyanobilin (PCB) is a chromophore necessary for this switch to work. PCB is the protein that senses light and changes its conformation to allow PhyB to bind to PIF3, as well as unbind. In its inactive conformation, noted as the Pr conformation, can absorb red light and change its conformation to the active conformation, noted as the Pfr conformation. In the active conformation, PCB can absorb far-red light to revert back to the inactive conformation. We wanted to make this process self-sufficient. Therefore if the yeast was able to synthesize the PCB itself, it would not have to always be supplemented.
Link to more details about Project 1

Project 2: Rapid & Reversible Protein Localization using PhyB-PIF3 System

Brief Description

DESCRIPTION ........................................
Link to more details about Project 2

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+Our project uses the PhyB-PIF3 system to create this fast-reversible switch.  Phycocyanobilin (PCB) is a chromophore necessary for this switch to work.  PCB is the protein that senses light and changes its conformation to allow PhyB to bind to PIF3, as well as unbind.  In its inactive conformation, noted as the Pr conformation, can absorb red light and change its conformation to the active conformation, noted as the Pfr conformation.  In the active conformation, PCB can absorb far-red light to revert back to the inactive conformation. We wanted to make this process self-sufficient. Therefore if the yeast was able to synthesize the PCB itself, it would not have to always be supplemented.
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 [[Team:MIT/Projects/Project1|Link to more details about Project 1]]