Team:LCG-UNAM-Mexico:Journals:Uriel
From 2009.igem.org
(Difference between revisions)
(→August 10, 2009) |
(→August 10, 2009) |
||
Line 245: | Line 245: | ||
2)buffer 10X | 2)buffer 10X | ||
3)MgCl2 | 3)MgCl2 | ||
+ | |||
+ | I'm going to do again the PCR for ogr and cox only from C-117 and as a control C-1a and DH5alpha the former control neither has a cox gene nor an ogr gene. | ||
+ | |||
+ | PCR Reactions: 1) 2) 3) 4) 5) 6) 7) 8) 9) | ||
+ | |||
+ | Water 23µL + + + + + + + + + | ||
+ | Buffer 10X 5µL + + + + + + + + + | ||
+ | MgCl2 2.5µL + + + + + + + + + | ||
+ | dNTP's 3.5µL + + + + + + + + + | ||
+ | Taq 1µL + + + + + + + + + | ||
+ | primer fw 2.5µL | ||
+ | primer rev 2.5µL | ||
+ | DNA tamplate 10µL | ||
== August 11, 2009 == | == August 11, 2009 == |
Revision as of 07:14, 20 October 2009