Team:Freiburg bioware/Notebook/April

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Latest revision as of 10:23, 20 October 2009

FREiGEM

April

24th April 12:30 - 18:00 o'clock Manuel

Literaturrecherche zu FokI.
Entstandene Ideen:

three oligo's: two for binding the monomers and one to create a dsDNA where FokI can bind.
two oligo's: one for binding one monomer, the second for binding the second monomer and to create dsDNA (one monomer has to be inactivated).
one oligo: both binding sites of the monomers on one oligo (also one monomer has to be inactivated).

The 196 C-terminale aminoacids of FokI were used from other groups (see reference 1). Primersequences are also provided in the publication.
Reference 1: Freiburg09_FokI_Zn-Finger_1996.pdf

29th april 21o'clock Rüdiger

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   class="t">Human
 Practice</span></a>
+    <ul>
+      <li><a href="#">D'oh! Something's missing here.</a>
+        <ul>
+          <li><a href="#">1</a> </li>
+          <li><a href="#">2</a> </li>
+          <li><a href="#">3</a> </li>
+        </ul>
        </li>
        <li><a href="#">4</a></li>
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   class="r"></span><span class="t">Notebook</span></a></li>
    <li><a
- href="https://2009.igem.org/Team:Freiburg_bioware/cloning1"><span
- class="l"></span><span class="r"></span><span
- class="t">Parts</span></a><ul>
-      <li><a
- href="https://2009.igem.org/Team:Freiburg_bioware/cloning1">Basic
-Parts</a></li>
-      <li><a
- href="https://2009.igem.org/Team:Freiburg_bioware/cloning">Composite
-Parts</a></li>
-    </ul></li>
-<li><a
   href="https://2009.igem.org/Team:Freiburg_bioware/Collaboration"><span
   class="l"></span><span class="r"></span><span