Team:Freiburg bioware/Notebook/April

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<h2 class="art-PostHeaderIcon-wrapper">April<span
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April
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<h3>24th April 12:30 - 18:00 o'clock Manuel</h3>
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<div style="text-align: left;">Literaturrecherche zu FokI.<br />
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Entstandene Ideen:
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<ul>
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  <li>three oligo's: two for binding the monomers and one to
 +
create a dsDNA where FokI can bind.</li>
 +
  <li>two oligo's: one for binding one monomer, the second for
 +
binding
 +
the second monomer and to create dsDNA (one monomer has to be
 +
inactivated).</li>
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  <li>one oligo: both binding sites of the monomers on one oligo
 +
(also one monomer has to be inactivated).</li>
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</ul>
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<br />
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The 196 C-terminale aminoacids of FokI were used from other groups (see
 +
reference 1). Primersequences are also provided in the publication.<br />
 +
Reference 1: Freiburg09_FokI_Zn-Finger_1996.pdf
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<br />
<br />
<br />
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<div style="text-align: left;">Text here</div>
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<h3><a name="29"></a>29th april 21o'clock
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R&uuml;diger</h3>
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Latest revision as of 10:23, 20 October 2009

FREiGEM

  April

24th April 12:30 - 18:00 o'clock Manuel

Literaturrecherche zu FokI.
Entstandene Ideen:
  • three oligo's: two for binding the monomers and one to create a dsDNA where FokI can bind.
  • two oligo's: one for binding one monomer, the second for binding the second monomer and to create dsDNA (one monomer has to be inactivated).
  • one oligo: both binding sites of the monomers on one oligo (also one monomer has to be inactivated).

The 196 C-terminale aminoacids of FokI were used from other groups (see reference 1). Primersequences are also provided in the publication.
Reference 1: Freiburg09_FokI_Zn-Finger_1996.pdf

29th april 21o'clock Rüdiger


contact:  freigem09@googlemail.com

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