Team:Freiburg bioware/Notebook/April

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FREiGEM

April

24th April 12:30 - 18:00 o'clock Manuel

Literaturrecherche zu FokI.
Entstandene Ideen:

three oligo's: two for binding the monomers and one to create a dsDNA where FokI can bind.
two oligo's: one for binding one monomer, the second for binding the second monomer and to create dsDNA (one monomer has to be inactivated).
one oligo: both binding sites of the monomers on one oligo (also one monomer has to be inactivated).

The 196 C-terminale aminoacids of FokI were used from other groups (see reference 1). Primersequences are also provided in the publication.
Reference 1: Freiburg09_FokI_Zn-Finger_1996.pdf

29th april 21o'clock Rüdiger

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-<h2 class="art-PostHeaderIcon-wrapper">April<span
+<h2 style="border-bottom: none;" class="art-PostHeaderIcon-wrapper"> &nbsp;<img
-  class="art-PostHeader"></span> </h2>
+  style="width: 28px; height: 25px;" alt=""
+ src="https://static.igem.org/mediawiki/2009/2/2a/Freiburg09_Post_tanne_2.png" />
+April
+<span class="art-PostHeader"></span> </h2>
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+<div class="art-PostContent">
+<div style="text-align: center;"></div>
+<h3>24th April 12:30 - 18:00 o'clock Manuel</h3>
+<div style="text-align: left;">Literaturrecherche zu FokI.<br />
+Entstandene Ideen:
+<ul>
+  <li>three oligo's: two for binding the monomers and one to
+create a dsDNA where FokI can bind.</li>
+  <li>two oligo's: one for binding one monomer, the second for
+binding
+the second monomer and to create dsDNA (one monomer has to be
+inactivated).</li>
+  <li>one oligo: both binding sites of the monomers on one oligo
+(also one monomer has to be inactivated).</li>
+</ul>
+<br />
+The 196 C-terminale aminoacids of FokI were used from other groups (see
+reference 1). Primersequences are also provided in the publication.<br />
+Reference 1: Freiburg09_FokI_Zn-Finger_1996.pdf
+<br />
 <br />
-<div style="text-align: left;">Text here</div>
+<h3><a name="29"></a>29th april 21o'clock
-<div style="text-align: left;"><br />
+R&uuml;diger</h3>
+<table style="text-align: left; width: 633px; height: 76px;"
+ border="0" cellpadding="0" cellspacing="2">
+  <tbody>
+    <tr>
+      <td><img style="width: 300px; height: 300px;"
+ alt=""
+ src="https://static.igem.org/mediawiki/2009/9/99/Freiburg09_huebsche_fokI.jpg" /></td>
+      <td><img style="width: 300px; height: 300px;"
+ alt=""
+ src="https://static.igem.org/mediawiki/2009/2/23/Freiburg09_huebsche_fokI_surface_render.jpg" /></td>
+    </tr>
+    <tr>
+      <td><img style="width: 300px; height: 300px;"
+ alt=""
+ src="https://static.igem.org/mediawiki/2009/6/6a/Freiburg09_fokI_3oligos.JPG" /></td>
+      <td><img style="width: 300px; height: 300px;"
+ alt=""
+ src="https://static.igem.org/mediawiki/2009/6/6e/Freiburg09_fokI_1inactive.JPG" /></td>
+    </tr>
+    <tr>
+      <td><img style="width: 300px; height: 300px;"
+ alt=""
+ src="https://static.igem.org/mediawiki/2009/8/8a/Freiburg09_fokI_1oligo.JPG" /></td>
+      <td><img style="width: 300px; height: 300px;"
+ alt=""
+ src="https://static.igem.org/mediawiki/2009/4/4a/Freiburg09_fokI_pna.JPG" /></td>
+    </tr>
+  </tbody>
+</table>
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