Team:Todai-Tokyo/Protocols/Miniprep

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# Use the Promega miniprep kit according to instructions
# Use the Promega miniprep kit according to instructions
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We re-suspend the DNA pellet in 50µl MilliQ and measure nucleotide concentration on a spectrophotometer to label on the tube.
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We usually elute the plasmid DNA from the spin column by 50µl MilliQ and measure nucleotide concentration on a spectrophotometer to label on the tube.

Revision as of 10:41, 20 October 2009

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Miniprep Protocol

Preparation (previous night)

  1. Pick Single colonies from a transformation plate and culture in 4ml Luria Broth
  2. Culture overnight at 37ºC with vigorous shaking

Miniprep

  1. Aliquot 2ml of the bacterial suspension from above into a 2ml eppendorf
  2. Spin down for 5 min. at max speed to pellet cells
  3. Use the Promega miniprep kit according to instructions

We usually elute the plasmid DNA from the spin column by 50µl MilliQ and measure nucleotide concentration on a spectrophotometer to label on the tube.

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