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From 2009.igem.org
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- 01:25, 22 October 2009 (diff | hist) Team:Todai-Tokyo/Modeling (→Modeling and the Result)
- 01:23, 22 October 2009 (diff | hist) Team:Todai-Tokyo/Modeling (→Construction of the Model)
- 01:13, 22 October 2009 (diff | hist) Team:Todai-Tokyo/Modeling (→Overview)
- 01:09, 22 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols (top)
- 16:20, 21 October 2009 (diff | hist) Team:Todai-Tokyo
- 16:13, 21 October 2009 (diff | hist) Team:Todai-Tokyo
- 14:20, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols/Infusion (→Infusion Protocol)
- 10:29, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols/Infusion (→Infusion Protocol)
- 10:27, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols/Infusion
- 10:18, 20 October 2009 (diff | hist) Jamboree/Schedule/Practice sessions (→Friday October 30 : Practice Talks sign-up sheet)
- 10:14, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols/Notebook Sample (top)
- 09:11, 20 October 2009 (diff | hist) N Team:Todai-Tokyo/Protocols/Infusion (New page: {{:Team:Todai-Tokyo/Template}} == Infusion Protocol ==)
- 09:10, 20 October 2009 (diff | hist) N Team:Todai-Tokyo/Protocols/Sequencing (New page: {{:Team:Todai-Tokyo/Template}} == Sequencing Protocol == We use the Big Dye Sequencing system with using the following conditions. # Prepare the following solution: ## 1.8ul 5 ｘ B.D....)
- 08:54, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols
- 08:52, 20 October 2009 (diff | hist) N Team:Todai-Tokyo/Protocols/Gel Extraction (New page: {{:Team:Todai-Tokyo/Template}} == Gel Purification Protocol == # Cut out volume of agarose gel containing the desired DNA and put in an eppendorf tube # Use the Promega Gel Purification...)
- 08:45, 20 October 2009 (diff | hist) N Team:Todai-Tokyo/Protocols/Colony PCR (New page: {{:Team:Todai-Tokyo/Template}} == Colony PCR Protocol == # Aliquot into PCR tubes 5µl of MilliQ # Pick single colonies with a toothpick and immerse in the above MilliQ to suspend # Put...)
- 08:10, 20 October 2009 (diff | hist) N Team:Todai-Tokyo/Protocols/PCR/programs (New page: {{:Team:Todai-Tokyo/Template}} == PCR Programs == === Pfu Standard === # 95ºC 2 min # 95ºC 30 sec # 52-55ºC 30 sec （Depending on the Tm of the primers） # 72.5ºC 15 sec × （# ...) (top)
- 07:52, 20 October 2009 (diff | hist) N Team:Todai-Tokyo/Protocols/PCR/Enzymes (New page: {{:Team:Todai-Tokyo/Template}} == Taq Polymerases == === Pfu Ultra === Unless otherwise stated, we use this Taq * 0.2µl 100µM 5’primer * 0.2µl 100µM 3’primer * 1.6µl 2....)
- 07:40, 20 October 2009 (diff | hist) N Team:Todai-Tokyo/Protocols/PCR (New page: {{:Team:Todai-Tokyo/Template}} == PCR Protocol == Our PCR protocol is a combination of Taq used and the PCR program.<BR> Please refer to the following pages for details on each. * [[Te...)
- 07:32, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols/Restriction Enzyme Digest
- 07:31, 20 October 2009 (diff | hist) N Team:Todai-Tokyo/Protocols/Restriction Enzyme Digest (New page: {{:Team:Todai-Tokyo/Template}} == Restriction Enzyme Digest Protocol == 1. Mix the following in a eppendorf tube: * Xµl plasmid DNA （to a final concentration of 300ng/30ul） * 3...)
- 06:56, 20 October 2009 (diff | hist) N Team:Todai-Tokyo/Protocols/Ligation (New page: {{:Team:Todai-Tokyo/Template}} == Ligation Protocol == # Mix 2µl of insert DNA with 3µl of vector DNA # Add 5µl Takara Ligation High # Incubate at 17ºC for 30 min.)
- 06:46, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols/Transformation (→Transformation Protocol)
- 06:45, 20 October 2009 (diff | hist) N Team:Todai-Tokyo/Protocols/Transformation (New page: {{:Team:Todai-Tokyo/Template}} == Transformation Protocol == '''From iGEM Plates''' <BR> # Add 15µl TE (Tris-EDTA, pH 8.0) to well containing part and pipette up and down to resuspend...)
- 06:34, 20 October 2009 (diff | hist) N Team:Todai-Tokyo/Protocols/Miniprep (New page: {{:Team:Todai-Tokyo/Template}} == Miniprep Protocol == '''Preparation''' (previous night)<BR> # Pick Single colonies from a transformation plate and culture in 4ml Luria Broth # Cultur...)
- 06:15, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols
- 05:22, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols/Notebook Sample
- 04:05, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols/Notebook Sample
- 03:26, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols/Notebook Sample
- 03:16, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols/Notebook Sample
- 03:00, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols/Notebook Sample
- 02:47, 20 October 2009 (diff | hist) N /Protocols/Miniprep (New page: test) (top)
- 02:44, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols/Notebook Sample
- 02:43, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols/Notebook Sample
- 02:35, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols/Notebook Sample
- 02:27, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols/Notebook Sample
- 02:26, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols/Notebook Sample
- 02:15, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols/Notebook Sample
- 02:12, 20 October 2009 (diff | hist) N Team:Todai-Tokyo/Protocols/Notebook Sample (New page: {{:Team:Todai-Tokyo/Template}} {| width="100%" cellpadding="0px" cellspacing="5px" style="font-family:'Century Gothic', sans-serif;" |- style="background:#BC8F8FF; height:100px;" valign="...)
- 02:12, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Notebook
- 02:10, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols
- 02:09, 20 October 2009 (diff | hist) /Notebook Sample (top)
- 02:09, 20 October 2009 (diff | hist) N /Notebook Sample (New page: {| width="100%" cellpadding="0px" cellspacing="5px" style="font-family:'Century Gothic', sans-serif;" |- style="background:#BC8F8FF; height:100px;" valign="top" |style="width:15%;"| <html...)
- 02:06, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Notebook
- 02:03, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Notebook/isoleucine (→Plan)
- 02:02, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Notebook
- 02:01, 20 October 2009 (diff | hist) Team:Todai-Tokyo/Notebook
- 15:14, 19 October 2009 (diff | hist) Team:Todai-Tokyo/Protocols
- 15:04, 19 October 2009 (diff | hist) N Team:Todai-Tokyo/Protocols (New page: Here is a list of common protocols we used for our projects.<BR> Individual protocols are linked from the Notebook section in order to minimize the amount of text in the Notebook. [/Mini...)
- 14:59, 19 October 2009 (diff | hist) Team:Todai-Tokyo/Template
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