"
Virginia Commonwealth/26 June 2009
From 2009.igem.org
(Difference between revisions)
(→Wetlab) |
(→Friday 26 June 2009) |
||
| Line 39: | Line 39: | ||
==Friday 26 June 2009== | ==Friday 26 June 2009== | ||
===Results=== | ===Results=== | ||
| - | *1 Plate growing pSB3K3 plasmid grew well. | + | *1 Plate growing pSB3K3 plasmid grew well. There was no fluorescence expressed. |
| - | *2 Plate growing the device plasmid pSB3K3, BBa_J23102, and E0240 grew well. | + | *2 Plate growing the device plasmid pSB3K3, BBa_J23102, and E0240 grew well. There was no fluorescence expressed. |
| + | |||
---- | ---- | ||
| Line 50: | Line 51: | ||
====Wetlab==== | ====Wetlab==== | ||
*1 IPTG stock was prepared. Since only 0.746g of IPTG stock was available, only 2.64 mL was prepared. - | *1 IPTG stock was prepared. Since only 0.746g of IPTG stock was available, only 2.64 mL was prepared. - | ||
| - | *2 IPTG stock (5.0 microliters) was added to 5 mL of bacteria that was grown overnight with pSB3k3 backbone (to induce the expression of RFP) | + | *2 IPTG stock (5.0 microliters) was added to 5 mL of bacteria that was grown overnight with pSB3k3 backbone (to induce the expression of RFP). After several hours no fluorescence was expressed. 3 mL LB+KAN was added to each culture so that the cells could re-enter log phase growth. 3 uL IPTG was also added to each culture to maintain concentration. |
Revision as of 18:04, 26 June 2009
|
|
|
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Contents |
Friday 26 June 2009
Results
- 1 Plate growing pSB3K3 plasmid grew well. There was no fluorescence expressed.
- 2 Plate growing the device plasmid pSB3K3, BBa_J23102, and E0240 grew well. There was no fluorescence expressed.
Tasks
- 1 Pick colonies and grow overnight.
- 2 Confirm the growing colonies using GFP and RFP reporter.
- 3 Order DNA
Wetlab
- 1 IPTG stock was prepared. Since only 0.746g of IPTG stock was available, only 2.64 mL was prepared. -
- 2 IPTG stock (5.0 microliters) was added to 5 mL of bacteria that was grown overnight with pSB3k3 backbone (to induce the expression of RFP). After several hours no fluorescence was expressed. 3 mL LB+KAN was added to each culture so that the cells could re-enter log phase growth. 3 uL IPTG was also added to each culture to maintain concentration.
